ABSTRACT
All over the world Hepatitis C virus [HCV] remains to be a main etiological mediator of liver disease. Approximately, 10 million people in Pakistan are diseased with HCV. Pegylated interferon plus ribavirin signifies the gold standard therapy but various side effects may occur. Comparative study. This study was conducted at Jinnah Hospital Lahore from August 2013 to March, 2014. Thirty five patients of hepatitis C virus and Twenty three age and sex-matched clinically apparently healthy individuals were eligible for inclusion in the study at Jinnah Hospital Lahore during the year 2013-2014. 1.0 ml blood sample were taken and subjected to centrifuge at 3000-4000 rpm for 10-15 minutes for the separation of serum. All the analytical work was performed at the Institute of molecular biology and biotechnology [IMBB], and Centre for research in molecular medicine [CRiMM], The University of Lahore-Pakistan. The estimation of AST, ALT, ALP, TP and T.Bilirubin were estimated. The AST level in HCV patients was increases [47.88 +/- 40.49] as compared to the control persons [31.43 +/- 7.31] and statistically significant [0.02<0.05]. Total Protein level in HCV patients was [4.20 +/- 0.61] and in healthy individuals [6.23 +/- 0.51] and statistically significant [0.000<0.05]. MDA level in HCV patients was increases remarkably [8.58 +/- 1.19] and in control persons [1.47 +/- 0.54] and it was statistically significant [0.000<0.05]. There is a relationship between oxidative stress and ALP, ALT, AST and Albumin. The results of the present study confirmed a perfect sketch regarding the circulating biochemical markers and lipid peroxidation [MDA] profile between the studied groups i.e., control and HCV patients with interferon induced Hepatitis C virus infection
ABSTRACT
Purpose of current study was to evaluate the effect of antiretroviral drugs [Three regimen] Ziduvodine, Lamivudine and Niverapine to HIV patients presented in D.G. Khan Zone in regard to CD4 level and viral loads before start of drugs and after one year treatment. Comparative study. This study was carried out at the Institute of Molecular Biology and Biotechnology, and Centre for Research in Molecular Medicine, The University of Lahore-Pakistan from April 2013 to June 2014. Seventy five [75] patients suffering from HIV and thirteen [13] control individuals were selected for the study. Rapid testing and ELISA screening were performed for identification of presence/absence of virus and antibodies respectively. Viral load and CD4+ absolute count were also evaluated by PCR and Multiset software respectively. All the analytical work was performed at the Institute of molecular biology and biotechnology [IMBB], and Centre for research in molecular medicine [CRiMM], The University of Lahore-Pakistan. Statistically highly significant difference [P=.000] was observed regarding viral load before and after the treatment in HIV patients receiving combination therapy, ART [antiretroviral therapy]. The viral load in control and HIV patients before and after the treatment was [0.00, 3.23x10[4]] and [0.00, 1.45x10[2]] respectively. Levels of CD4+cells were increased and a highly significant difference was recorded among control and ART treated patients. Inverse correlation was recorded between viral loads [After] and CD4+ levels [After], [Viral Load Vs CD4+, r = -.678**]. It can be concluded that ART is highly effective in AIDS patients with minimal adverse effects, makes the life of AIDS patients less miserable and improve the quality of life
ABSTRACT
Purpose of current study was to evaluate the effect of antiretroviral drugs [Three regimen] Tenofovir, Lamivudine and Efavirenz to HIV patients presented in D.G. Khan Zone in regard to CD4 level and viral loads before start of drugs and after one year treatment. Comparative study. This study was carried out at the Institute of molecular biology and biotechnology [IMBB], and Centre for research in molecular medicine [CRiMM], The University of Lahore-Pakistan during May 2013 to Feb. 2014. Seventy five [75] patients suffering from HIV and twelve [12] control individuals were selected for the study from Dera Ghazi Khan during 2013-2014. Rapid testing and ELISA screening were performed for identification of presence/absence of virus and antibodies respectively. Viral load and CD4+ absolute count were also evaluated by PCR and Multiset software respectively. All the analytical work was performed at the Institute of molecular biology and biotechnology [IMBB], and Centre for research in molecular medicine [CRiMM], The University of Lahore-Pakistan. Statistically highly significant difference [P=.000] was observed regarding viral load before and after the treatment in HIV patients receiving combination therapy, ART [antiretroviral therapy]. The viral load in control and HIV patients before and after the treatment was [0.00, 3.22x10[4]] and [0.00, 1.61x10[2]] respectively. The CD4+ cells levels in control and HIV patients before and after the treatment was [330.67, 186.29] and [171.92, 372.64] respectively. Inverse correlation was also recorded between viral loads [After] and CD4+ levels [After], [Viral Load Vs CD4+, r=-.328**]. It is concluded that from one year antiviral therapy in AIDS patients the viral load decrease from 3.22x10[4] to 1.61x10[2] and CD4+ count increased from 186.29 to 372.64 with no significant complications hence improve the AIDS patients' lives and minimize the spread of infection